Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: RT-qPCR analysis of DNMTs and IL1R2 expression, along with IHC staining (A) RT-qPCR was used to quantitatively analyze the expression profiles of DNMTs and IL1R2 in HBW and LBW placentas ( DNMT3A : p = 0.0264; DNMT3L : p = 0.0016; DNMT1 : p = 0.0069; IL1R2 : p = 0.0156). (B and D) IHC was performed to analyze the protein expression intensity of DNMT3A in HBW and LBW placentas (DNMT3A: p = 0.0059). Scale bars, 50 μm. (C and E) IHC was performed to analyze the protein expression intensity of IL1R2 in HBW and LBW placentas (IL1R2: p = 0.0007). Scale bars, 50 μm. Data are represented as mean ± SEM. Statistical significance was assessed by paired Student’s t test, with n = 6 (3 samples in the LBW group and 3 samples in the HBW group).

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Quantitative RT-PCR, Expressing, Immunohistochemistry

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: MethPrimer-based prediction of CpG islands in the IL1R2 promoter region and subsequent analysis of the methylation status in placental tissues (A) Bioinformatics analysis predicted CpG islands in the promoter region of IL1R2 . (B) MS-PCR measured IL1R2 methylation levels in HBW and LBW placentas ( p = 0.0062). Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, with n = 6 (3 samples in the LBW group and 3 samples in the HBW group).

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Methylation

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: Effects of 5-Aza treatment on IL1R2 gene expression and methylation levels in PTr2 cells (A) The mRNA expression of IL1R2 in 5-Aza-treated PTr2 cells was measured using RT-qPCR (20 μM: p = 0.0199). “ns” denotes non-significant results ( p > 0.05). (B) MS-PCR measured the methylation levels of the IL1R2 promoter in 5-Aza-treated PTr2 Cells ( p = 0.0005). (C) BS-PCR measured the methylation levels of the IL1R2 promoter in 5-Aza-treated PTr2 cells ( p = 0.0011). Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Gene Expression, Methylation, Expressing, Quantitative RT-PCR

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: IL1R2 knockdown promotes PTr2 Cell proliferation and migration (A) RT-qPCR analysis of IL1R2 knockdown efficiency and its effect on proliferation and apoptosis markers in PTr2 cells ( IL1R2 : p = 0.0002; Ki67 : p = 0.0043; BAX : p = 0.0155; CASP3 : p = 0.0088; CASP9 : p = 0.0331). “ns” denotes non-significant results ( p > 0.05). (B) Cell proliferation assessed using CCK-8 assay after IL1R2 knockdown (48 h: p = 0.0224; 72 h: p = 0.0035). (C and D) Cell proliferation evaluated using EdU assay ( p = 0.0083). Scale bars, 100 μm. (E and F) Cell migration was analyzed by scratch assay ( p = 0.0462). Scale bars, 500 μm. Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Knockdown, Migration, Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Wound Healing Assay

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: IL1R2 overexpression inhibits PTr2 Cell proliferation and migration (A) RT-qPCR analysis of IL1R2 overexpression efficiency and its effect on proliferation and apoptosis markers in PTr2 cells ( IL1R2 : p = 0.0003; PCNA : p = 0.0256; BAX : p = 0.0497). “ns” denotes non-significant results ( p > 0.05). (B) Cell proliferation was assessed using CCK-8 assay following IL1R2 overexpression (48 h: p = 0.0027; 72 h: p = 0.0011). (C and D) Cell proliferation evaluated using EdU assay ( p = 0.0452). Scale bars, 100 μm. (E and F) Cell migration was analyzed by scratch assay ( p = 0.0078). Scale bars, 500 μm. Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Over Expression, Migration, Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Wound Healing Assay

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: IL1R2 positively regulates TNF- α expression in PTr2 cells (A) IL1R2 knockdown suppresses TNF-α mRNA levels ( p = 0.0442). (B) IL1R2 overexpression elevates TNF-α mRNA levels ( p = 0.0057). (C - E) IL1R2 knockdown reduces TNF- α protein abundance, with confirmation of knockdown efficiency (IL1R2: p = 0.0012; TNF-α: p = 0.0394). (F - H) IL1R2 overexpression increases TNF- α protein levels, with confirmation of knockdown efficiency (IL1R2: p = 0.0011; TNF-α: p = 0.0014). Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Expressing, Knockdown, Over Expression, Quantitative Proteomics

Journal: Chinese Medicine

Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway

doi: 10.1186/s13020-026-01387-z

Figure Lengend Snippet: Effects of matrine on intestinal pathology, inflammatory function, and JAK2 expression in experimental colitis mice Dextran Sulfate Sodium (DSS)-induced murine model of colitis was established in mice; matrine and 5-ASA treatment was administered as described. A – C Body weight and colon length were measured. D , E Histopathological alterations in colon tissues were evaluated using H & E staining; DAI scores were assigned according to the staining. F , G The levels of IL-1β, TNF-α, IL-6, MPO, NO, and MDA in colon tissues were examined using commercial assay kits. H , I The protein levels of JAK2 in mice colon tissues were determined using Immunohistochemical staining (IHC staining) and Immunoblotting. Magnification = 100 × or 200 × for IHC staining. J The protein levels of p-JAK2, p-STAT3, and STAT3 were detected using Immunoblotting. **p < 0.01, vs. PBS group; #p < 0.05, ##p < 0.01 vs. DSS group

Article Snippet: Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), IL-1β [12242, Cell Signaling Technology (CST), Danvers, USA], TNF-α (11948, CST), IL-6 (CSB-PA06757A0RB, CUSABIO, Wuhan, China), p-STAT3 (AF3293, Affinity Bioscience), STAT3 (10253-2-AP, Proteintech, Wuhan, China), p-STAT1 (28977-1-AP, Protientech), STAT1 (10144-2-AP, Proteintech), FXR (M022312, Abmart, Shanghai, China), and GAPDH (endogenous control, 60004-1-Ig, Proteintech) overnight at 4 °C (dilution 1:1000).

Techniques: Expressing, Staining, Immunohistochemical staining, Immunohistochemistry, Western Blot

Journal: Chinese Medicine

Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway

doi: 10.1186/s13020-026-01387-z

Figure Lengend Snippet: Effects of JAK2 overexpression on intestinal epithelial cells upon inflammation MODE-K cells were transfected with a JAK2-overexpressing plasmid (JAK2 oe) or a control vector, with or without subsequent LPS stimulation. A The protein levels of JAK2 and p-JAK2 and the inflammatory factors IL-1β, TNF-α, and IL-6 were determined by Immunoblotting. B Cellular levels of MPO, NO, and MDA were measured using biochemical kits. C Intracellular ROS production was assessed by DCFH-DA staining and quantified with flow cytometry. D The protein levels of the bile acid metabolism-related factors FXR, MRP3 and MRP4 were examined by Immunoblotting. E The mRNA levels of MRP3, MRP4, OSTα, and OSTβ were measured by qRT-PCR. **p < 0.01 vs. Vector group; ##p < 0.01 vs. Vector group; &&p < 0.01 vs. LPS + Vector group

Article Snippet: Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), IL-1β [12242, Cell Signaling Technology (CST), Danvers, USA], TNF-α (11948, CST), IL-6 (CSB-PA06757A0RB, CUSABIO, Wuhan, China), p-STAT3 (AF3293, Affinity Bioscience), STAT3 (10253-2-AP, Proteintech, Wuhan, China), p-STAT1 (28977-1-AP, Protientech), STAT1 (10144-2-AP, Proteintech), FXR (M022312, Abmart, Shanghai, China), and GAPDH (endogenous control, 60004-1-Ig, Proteintech) overnight at 4 °C (dilution 1:1000).

Techniques: Over Expression, Transfection, Plasmid Preparation, Control, Western Blot, Staining, Flow Cytometry, Quantitative RT-PCR

Journal: Chinese Medicine

Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway

doi: 10.1186/s13020-026-01387-z

Figure Lengend Snippet: Effects of matrine on intestinal epithelial cell function upon inflammation Mouse intestinal epithelial cell line, MODE-K, was stimulated with LPS with or without matrine treatment (1, 2, 3 mg/ml), and examined for the protein levels of IL-1β, TNF-α, and IL-6 using Immunoblotting ( A ); The levels of MPO, NO, and MDA using commercial kits ( B ); Intracellular ROS levels were detected by flow cytometry ( C ); The protein levels of FXR, MRP3 and MRP4 were examined using Immunoblotting ( D ); The mRNA levels of MRP3, MRP4, OSTα and OSTβ were examined using qRT-PCR ( E ); The protein levels of p-JAK2, JAK2, p-STAT3, STAT3, p-STAT1, and STAT1 using Immunoblotting ( F ). **p < 0.01, vs. PBS group; #p < 0.05, ##p < 0.01 vs. LPS group

Article Snippet: Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), IL-1β [12242, Cell Signaling Technology (CST), Danvers, USA], TNF-α (11948, CST), IL-6 (CSB-PA06757A0RB, CUSABIO, Wuhan, China), p-STAT3 (AF3293, Affinity Bioscience), STAT3 (10253-2-AP, Proteintech, Wuhan, China), p-STAT1 (28977-1-AP, Protientech), STAT1 (10144-2-AP, Proteintech), FXR (M022312, Abmart, Shanghai, China), and GAPDH (endogenous control, 60004-1-Ig, Proteintech) overnight at 4 °C (dilution 1:1000).

Techniques: Cell Function Assay, Western Blot, Flow Cytometry, Quantitative RT-PCR

Journal: Chinese Medicine

Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway

doi: 10.1186/s13020-026-01387-z

Figure Lengend Snippet: Dynamic effects of matrine and JAK2 on intestinal epithelial cell function upon inflammation MODE-K cells were stimulated with LPS with or without matrine treatment (2 mg/ml), transfected with JAK2-overexpressing plasmid (JAK2 oe), and examined for the protein levels of IL-1β, TNF-α, and IL-6 using Immunoblotting ( A ); the levels of MPO, NO, and MDA using commercial kits ( B ); Intracellular ROS levels were detected by flow cytometry ( C ); The protein levels of FXR, MRP3 and MRP4 were examined using Immunoblotting ( D ); The mRNA levels of MRP3, MRP4, OSTα and OSTβ were examined using qRT-PCR ( E ); the protein levels of JAK2, p-STAT3, STAT3, p-STAT1, and STAT1 using Immunoblotting ( F ). **p < 0.01, vs. LPS group; #p < 0.05, ## p < 0.01 vs. LPS + matrine + vector group

Article Snippet: Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), IL-1β [12242, Cell Signaling Technology (CST), Danvers, USA], TNF-α (11948, CST), IL-6 (CSB-PA06757A0RB, CUSABIO, Wuhan, China), p-STAT3 (AF3293, Affinity Bioscience), STAT3 (10253-2-AP, Proteintech, Wuhan, China), p-STAT1 (28977-1-AP, Protientech), STAT1 (10144-2-AP, Proteintech), FXR (M022312, Abmart, Shanghai, China), and GAPDH (endogenous control, 60004-1-Ig, Proteintech) overnight at 4 °C (dilution 1:1000).

Techniques: Cell Function Assay, Transfection, Plasmid Preparation, Western Blot, Flow Cytometry, Quantitative RT-PCR